Articles

Dr. Ayal Hendel

Dr. Hendel Ayal – Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System

Genome editing of human cluster of differentiation 34+ (CD34+) hematopoietic stem and progenitor cells (HSPCs) holds great therapeutic potential. This study aimed to optimize on-target, ex vivo genome editing using the CRISPR-Cas9 system in CD34+ HSPCs and to create a clear workflow for precise identification of off-target effects. Modified synthetic guide RNAs (gRNAs), either 2-part gRNA or single-guide RNA (sgRNA),…

Prof. Arnon Blum – Vascular Reactivity of Adult-Onset Systemic Lupus Erythematosus Patients

Abstract Background: Cardiovascular disease (CVD) is more frequent in patients with systemic lupus erythematosus (SLE) compared with age- and sex-matched healthy subjects. SLE is an autoimmune disease that is more prevalent in women (9:1). Women tend to develop CVD in post-menopausal years; however, women with SLE may develop endothelial dysfunction and CVD at a younger age…

Dr. Yissachar Nissan – Ménage à trois: regulation of host immunity by enteric neuro-immune-microbiota cross talks

Highlights • The enteric nervous and immune systems closely interact to control gut homeostasis. • The gut microbiota impact enteric neuroimmune communications and host immunity. • Microbial dysbiosis disturbs homeostatic enteric innervation and immunocyte activity. • The enteric neuro-immune-microbiota interface impact local and systemic physiology. Over the past decade, our understanding of intestinal immunology has…

Dr. Yissachar Nissan – β-arrestin 2 quenches TLR signaling to facilitate the immune evasion of EPEC

The protein translocated intimin receptor (Tir) from enteropathogenic Escherichia coli shares sequence similarity with the host cellular immunoreceptor tyrosine-based inhibition motifs (ITIMs). The ITIMs of Tir are required for Tir-mediated immune inhibition and evasion of host immune responses. However, the underlying molecular mechanism by which Tir regulates immune inhibition remains unclear. Here we demonstrated that β-arrestin 2,…
Prof. Erez Levanon

Prof. Levanon Erez – The cell line A-to-I RNA editing catalogue

Abstract Adenosine-to-inosine (A-to-I) RNA editing is a common post transcriptional modification. It has a critical role in protecting against false activation of innate immunity by endogenous double stranded RNAs and has been associated with various regulatory processes and diseases such as autoimmune and cardiovascular diseases as well as cancer. In addition, the endogenous A-to-I editing…
Dr. Ayal Hendel

Dr. Hendel Ayal – The cell line A-to-I RNA editing catalogue

Abstract Adenosine-to-inosine (A-to-I) RNA editing is a common post transcriptional modification. It has a critical role in protecting against false activation of innate immunity by endogenous double stranded RNAs and has been associated with various regulatory processes and diseases such as autoimmune and cardiovascular diseases as well as cancer. In addition, the endogenous A-to-I editing…

Prof. Arnon Blum – Sleep disorders and vascular responsiveness in patients with rheumatoid arthritis

Abstract Background Rheumatoid arthritis (RA) is the most common systemic autoimmune disease characterized by chronic systemic inflammation. Half of the deaths of patients with RA are due to cardiovascular diseases (CVD), considered to be 1.5 to −2.0‐fold that in the general population. Patients with RA also experience poor sleep, which by itself is associated with…

Prof. Noa Vilchinsky – Providing Care From Afar: A Growing Yet Understudied Phenomenon in the Caregiving Field

Given the aging population and increasing life expectancy, the need on the part of older and ill populations for long-term care has risen rapidly (Roth et al., 2015). As a result, unpaid informal care is becoming even more important for health and social care delivery worldwide. Informal care refers to the provision of unpaid care…

Prof. Rachel Dekel – Male Intimate Partner Violence: Examining the Roles of Childhood Trauma, PTSD Symptoms, and Dominance

Abstract One approach to understanding perpetration of intimate partner violence (IPV) by men focuses upon their childhood exposure to abuse or neglect as traumatic experiences, which may lead to PTSD symptoms; these symptoms can serve as risk factors for IPV perpetration. Another approach looks at the societal aspects of inequality between men and women as promoting male…

Dr. Dana Atzil-Slonim – Therapeutic Technique Diversity is Linked to Quality of Working Alliance and Client Functioning Following Alliance Ruptures

Abstract Objective: The current investigation aimed to examine the possible association between therapists’ flexibility in use of therapeutic techniques from different therapy orientations (i.e. therapeutic technique diversity; TTD) and subsequent improvement in client-reported (a) global functioning, as well as (b) quality of the working alliance, following sessions in which alliance ruptures occurred. Method: Clients (n…
Dr. Ayal Hendel

Dr. Hendel Ayal – CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells

Abstract The prototypical genetic autoimmune disease is immune dysregulation polyendocrinopathy enteropathy X-linked (IPEX) syndrome, a severe pediatric disease with limited treatment options. IPEX syndrome is caused by mutations in the forkhead box protein 3 (FOXP3) gene, which plays a critical role in immune regulation. As a monogenic disease, IPEX is an ideal candidate for a…

Prof. Noa Vilchinsky – Early Exposure to Cardiac Treatment and Distress Among Patients and Their Caregiving Partners

Background: The experience of an acute coronary event (ACE), including early care and evaluation, can be a distressing and traumatic experience for patients and their romantic partners, who also act as caregivers. We hypothesized that, among partners who were present during the ACE, those who were also present during (1) transportation to the hospital and (2)…

Dr. Hava Gil-Henn – Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer’s Disease Mice

Abstract Adult neurogenesis is a complex physiological process, which plays a central role in maintaining cognitive functions, and consists of progenitor cell proliferation, newborn cell migration, and cell maturation. Adult neurogenesis is susceptible to alterations under various physiological and pathological conditions. A substantial decay of neurogenesis has been documented in Alzheimer’s disease (AD) patients and…

Prof. Noa Vilchinsky – Attachment insecurity hinders cardiac patients’ ability to receive partners’ care: A longitudinal dyadic study

Attachment insecurity hinders cardiac patients’ ability to receive partners’ care: A longitudinal dyadic study Show all authorsSivan George-Levi, Shira Peleg, Noa Vilchinsky, …First Published February 3, 2020 Research Articlehttps://doi.org/10.1177/0265407519900998 Article information  Abstract Objectives: The fact that spousal support is not always beneficial for the recipient continues to intrigue researchers in the dyadic support field. One possible explanation for this phenomenon…

Prof. Ron Unger – Cervical dilation at the time of epidural catheter insertion is not associated with the degree of prolongation of the first or second stages of labor, or the rate of instrumental vaginal delivery

Abstract Introduction Epidural analgesia (EA) is an established option for efficient intrapartum analgesia. Meta‐analyses have shown that EA differentially affects the first stage of labor but prolongs the second. The question of EA timing remains open. We aimed to investigate whether EA prolongs delivery in total and whether the EA administration timing vis‐à‐vis cervical dilation…

Prof. Ron Unger – Prediction of vaginal birth after cesarean deliveries using machine learning

Background Efforts to reduce cesarean delivery rates to 12–15% have been undertaken worldwide. Special focus has been directed towards parturients who undergo a trial of labor after cesarean delivery to reduce the burden of repeated cesarean deliveries. Complication rates are lowest when a vaginal birth is achieved and highest when an unplanned cesarean delivery is…
Dr. Milana Frenkel-Morgenstern

Dr. Milana Frenkel-Morgenstern – Current Perspectives on Circulating Tumor DNA, Precision Medicine, and Personalized Clinical Management of Cancer

Abstract Circulating tumor DNA (ctDNA) has recently emerged as a minimally invasive ‘liquid biopsy’ tool in precision medicine. ctDNA-genomic DNA fragments that are released into the bloodstream after the active secretion of microvesicles or tumor cell lysis-reflects tumor evolution and the genomic alterations present in primary and/or metastatic tumors. Notably, ctDNA analysis might allow the…

Dr. Orly Avni – Schistosomal extracellular vesicle-enclosed miRNAs modulate host T helper cell differentiation

Abstract During the chronic stage of Schistosoma infection, the female lays fertile eggs, triggering a strong anti‐parasitic type 2 helper T‐cell (Th2) immune response. It is unclear how this Th2 response gradually declines even though the worms live for years and continue to produce eggs. Here, we show that Schistosoma mansoni downregulates Th2 differentiation in an antigen‐presenting cell‐independent manner,…

Prof. Cyrille Cohen – A TIGIT-based chimeric co-stimulatory switch receptor improves T-cell anti-tumor function

Abstract Background Tumors can employ different mechanisms to evade immune surveillance and function. Overexpression of co-inhibitory ligands that bind to checkpoint molecules on the surface of T-cells can greatly impair the function of latter. TIGIT (T cell immunoreceptor with Ig and ITIM domains) is such a co-inhibitory receptor expressed by T and NK cells which, upon…

Prof. Rachel Dekel – The role of complex posttraumatic stress symptoms in the association between exposure to traumatic events and severity of intimate partner violence

Abstract Background Symptoms of both posttraumatic stress disorder (PTSD) and disturbances in self-organization (DSO) have been suggested to play a role in the association between an individual’s childhood physical abuse and neglect and his/her perpetration of IPV in adulthood; however, the two have yet to be studied in one model. Thus, we aimed to examine…
Prof. Nirit Bauminger-Zviely

Prof. Nirit Bauminger-Zviely – Preschool Peer Social Intervention (PPSI) to Enhance Social Play, Interaction, and Conversation: Study Outcomes

Abstract This RCT study examined efficacy of a preschool peer social intervention (PPSI) in facilitating social engagement of preschoolers with high-functioning ASD (HFASD; N = 65). HFASD participants were randomly assigned by preschool to a 6-month intervention (play, interaction, or conversation) or a waitlisted-treatment-as-usual control group. Trained on-site therapists led the PPSI in preschools, in small (n = 3–4) mixed…
Dr. Milana Frenkel-Morgenstern

Dr. Milana Frenkel-Morgenstern – ChiTaRS 5.0: the comprehensive database of chimeric transcripts matched with druggable fusions and 3D chromatin maps

Abstract Chimeric RNA transcripts are formed when exons from two genes fuse together, often due to chromosomal translocations, transcriptional errors or trans-splicing effect. While these chimeric RNAs produce functional proteins only in certain cases, they play a significant role in disease phenotyping and progression. ChiTaRS 5.0 (http://chitars.md.biu.ac.il/) is the latest and most comprehensive chimeric transcript…

Dr. Hava Gil-Henn – Invasion and metastasis: the elusive hallmark of cancer

In their seminal review published nearly two decades ago, Hanahan and Weinberg introduced the “hallmarks of cancer” and typified essential biological abilities that are acquired by human cancer [1], including sustained proliferative signaling, resistance to apoptosis, evading growth suppressors, induction of angiogenesis, enabling replicative immortality, and invasion and metastasis. Eleven years later, in 2011, the…
Dr. Milana Frenkel-Morgenstern

Dr. Milana Frenkel-Morgenstern – Identification of Chimeric RNAs Using RNA-Seq Reads and Protein–Protein Interactions of Translated Chimeras

Abstract Chimeric RNA moieties typically consist of exons from two genes expressed from different genomic locations and produced by chromosomal translocations, trans-splicing or transcription errors. Recent advances in next-generation sequencing procedures have opened new horizons for identification of novel chimeric transcripts in various diseases in a personalized manner. Here we describe the detailed computational procedures to…
Prof. Erez Levanon

Prof. Levanon Erez – Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course

Abstract Ohtahara syndrome, early infantile epileptic encephalopathy with a suppression burst EEG pattern, is an aetiologically heterogeneous condition starting in the first weeks or months of life with intractable seizures and profound developmental disability. Using whole exome sequencing, we identified biallelic DMXL2 mutations in three sibling pairs with Ohtahara syndrome, belonging to three unrelated families. Siblings in…

Dr. Hava Gil-Henn – Hepatitis C Virus Enhances the Invasiveness of Hepatocellular Carcinoma via EGFR-Mediated Invadopodia Formation and Activation

Abstract Hepatocellular carcinoma (HCC) represents the fifth most common cancer worldwide and the third cause of cancer-related mortality. Hepatitis C virus (HCV) is the leading cause of chronic hepatitis, which often results in liver fibrosis, cirrhosis, and eventually HCC. HCV is the most common risk factor for HCC in western countries and leads to a…

Prof. Rachel Dekel – Posttraumatic Stress Disorder Upon Admission to Shelters Among Female Victims of Domestic Violence: An Ecological Model of Trauma

Abstract The study used the ecological model of trauma and recovery (Harvey, 1996) to examine the rates of probable posttraumatic stress disorder (PTSD) among female victims of domestic violence. Five hundred and five participants completed questionnaires upon entering shelters in Israel. Analysis showed that 61% of the participants reported probable PTSD. Childhood exposure to violence,…
Prof. Erez Levanon

Prof. Levanon Erez – Genome-wide quantification of ADAR adenosine-to-inosine RNA editing activity

Abstract Adenosine-to-inosine (A-to-I) RNA editing by the adenosine deaminase that acts on RNA (ADAR) enzymes is a common RNA modification, preventing false activation of the innate immune system by endogenous double-stranded RNAs. Methods for quantification of ADAR activity are sought after, due to an increasing interest in the role of ADARs in cancer and autoimmune…

Dr. Evan Elliott – Gene network analysis reveals a role for striatal glutamatergic receptors in dysregulated riskassessment behavior of autism mouse models

Abstract Autism spectrum disorder (ASD) presents a wide, and often varied, behavioral phenotype. Improper assessment of risks has been reported among individuals diagnosed with ASD. Improper assessment of risks may lead to increased accidents and self-injury, also reported among individuals diagnosed with ASD. However, there is little knowledge of the molecular underpinnings of the impaired…

Prof. Nirit Bauminger-Zviely – Communicating Without Words: School-Based RCT Social Intervention in Minimally Verbal Peer Dyads with ASD

Abstract Despite their social withdrawal, school-age children with autism spectrum disorder who are minimally verbal (MVASD; i.e., use a limited repertoire of communicative spoken words) have received few interventions supporting peer engagement. This study examined efficacy of a novel ecological randomized controlled trial—school-based peer social intervention—designed to increase social engagement (via available communication channels) in…

Dr. Meital Gal-Tanamy – Hepatitis C Virus Enhances the Invasiveness of Hepatocellular Carcinoma via EGFR-Mediated Invadopodia Formation and Activation

Abstract Hepatocellular carcinoma (HCC) represents the fifth most common cancer worldwide and the third cause of cancer-related mortality. Hepatitis C virus (HCV) is the leading cause of chronic hepatitis, which often results in liver fibrosis, cirrhosis, and eventually HCC. HCV is the most common risk factor for HCC in western countries and leads to a…
Prof. Rachela Popovtzer

Prof. Popovtzer Rachela – Intranasal Delivery of Mesenchymal Stem Cell Derived Exosomes Loaded with Phosphatase and Tensin Homolog siRNA Repairs Complete Spinal Cord Injury

Abstract Individuals with spinal cord injury (SCI) usually suffer from permanent neurological deficits, while spontaneous recovery and therapeutic efficacy are limited. Here, we demonstrate that when given intranasally, exosomes derived from mesenchymal stem cells (MSC-Exo) could pass the blood brain barrier and migrate to the injured spinal cord area. Furthermore, MSC-Exo loaded with phosphatase and…
Dr. Milana Frenkel-Morgenstern

Dr. Milana Frenkel-Morgenstern – ProtFus: A Comprehensive Method Characterizing Protein-Protein Interactions of Fusion Proteins

Abstract Tailored therapy aims to cure cancer patients effectively and safely, based on the complex interactions between patients’ genomic features, disease pathology and drug metabolism. Thus, the continual increase in scientific literature drives the need for efficient methods of data mining to improve the extraction of useful information from texts based on patients’ genomic features.…

Prof. Arnon Blum – MicroRNA-423 may regulate diabetic vasculopathy

Abstract To test the hypothesis that microRNAs may play a role in diabetic retinopathy, we measured the levels of different markers [microRNAs, vascular endothelial growth factor (VEGF), nitric oxide (NO), and total antioxidant capacity (TAO)] in patients with type 2 diabetes mellitus (T2DM) and microvascular complications. Sixty-nine patients were recruited: 22 healthy subjects, ten T2DM…

Dr. Omry Koren – Deep microbial analysis of multiple placentas shows no evidence for a placental microbiome

Abstract Objectives To resolve the controversy regarding the presence of a microbiota in the placenta. Design Classical and molecular microbiological study. Setting All samples were collected during caesarean section. Population A total of 28 human placentas and six murine placentas. Methods All 28 human placentas were checked for 16S rRNA gene amplification products. Three locations…
Dr. Ayal Hendel

Dr. Hendel Ayal – Reduced Function and Diversity of T Cell Repertoire and Distinct Clinical Course in Patients With IL7RA Mutation

Abstract The alpha subunit of IL-7 receptor (IL7R7α) is critical for the differentiation of T cells, specifically for the development and maintenance of γδT cells. Mutations in IL7RA are associated with Severe Combined Immunodeficiency (SCID). Infants with IL7RA deficiency can be identified through newborn screening program. We aimed at defining the immunological and genetic parameters that are directly affected…

ADr. Hava Gil-Henn – A helical lock and key model of polyproline II conformation with SH3

Abstract Motivation More than half of the human proteome contains the proline-rich motif, PxxP. This motif has a high propensity for adopting a left-handed polyproline II (PPII) helix and can potentially bind SH3 domains. SH3 domains are generally grouped into two classes, based on whether the PPII binds in a positive (N-to-C terminal) or negative…

Dr. Itay Onn – The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans

Abstract Chd1 is a chromatin remodeler that is involved in nucleosome positioning and transcription. Deletion of CHD1 is a frequent event in prostate cancer. The Structural Maintenance of Chromosome (SMC) complex cohesin mediates long-range chromatin interactions and is involved in maintaining genome stability. We provide new evidence that Chd1 is a regulator of cohesin. In…

Dr. Meital Gal-Tanamy – Hepatitis C virus leaves an epigenetic signature post cure of infection by directacting antivirals

Abstract The increasing worldwide prevalence of Hepatocellular carcinoma (HCC), characterized by resistance to conventional chemotherapy, poor prognosis and eventually mortality, place it as a prime target for new modes of prevention and treatment. Hepatitis C Virus (HCV) is the predominant risk factor for HCC in the US and Europe. Multiple epidemiological studies showed that sustained…

Prof. Ron Unger – Contemporary patterns of labor in nulliparous and multiparous women

Background Controversy surrounds the definition of “normal” and “abnormal” labor. Objective In this study, we used contemporary labor charts to explore labor patterns in large obstetric population (2011–2016). Study Design Detailed information from electronic medical records of live singleton deliveries at term (≥37 weeks of gestation) was extracted. Cases of elective cesarean deliveries, nonvertex presentation,…

Dr. Omry Koren – Modulation of cytokine patterns and microbiome during pregnancy in IBD

Abstract Objective Pregnancy may affect the disease course of IBD. Both pregnancy and IBD are associated with altered immunology and intestinal microbiology. However, to what extent immunological and microbial profiles are affected by pregnancy in patients with IBD remains unclear. Design Faecal and serum samples were collected from 46 IBD patients (31 Crohn’s disease (CD) and 15…
Dr. Ayal Hendel

Dr. Ayal Hendel – Primary immunodeficiencies – from early diagnosis and gene discovery to personalized therapy

Biology and medicine are undergoing a tremendous revolution where the use of advanced technologies, sophisticated bioinformatics and big data, revealing immediate impact on translation to medicine including disease diagnosis, treatment and outcome. Thus the main objective of this proposal that combines clinical and scientific skills is to extend our knowledge on pathways controlling human immunity…

Prof. Michaeli Shulamit

Nucleic Acids Research, 2019 1–15 doi: 10.1093/nar/gkz477 Pseudouridines on Trypanosoma brucei spliceosomal small nuclear RNAs and their implication for RNA and protein interactions K. Shanmugha Rajan1, Tirza Doniger 1, Smadar Cohen-Chalamish1, Dana Chen1, Oz Semo1, Saurav Aryal1, Efrat Glick Saar2, Vaibhav Chikne1, Doron Gerber 1, Ron Unger1, Christian Tschudi3 and Shulamit Michaeli 1,* 1The Mina…

Prof. Arnon Blum – Gender differences in cardiovascular risk of patients with rheumatoid arthritis

Abstract Background Rheumatoid Arthritis (RA) is a chronic inflammatory disease, affecting women more than men, with a more aggressive course in women. Design A prospective study that recruited 58 patients (46 women aged 56 ± 12 years) with active long-standing RA disease (>12 months). Our goals were to measure their endothelial function, part of the…

Prof. Cyrille Cohen – Level of neo-epitope predecessor and mutation type determine T cell activation of MHC binding peptides

Abstract Background Targeting epitopes derived from neo-antigens (or “neo-epitopes”) represents a promising immunotherapy approach with limited off-target effects. However, most peptides predicted using MHC binding prediction algorithms do not induce a CD8 + T cell response, and there is a crucial need to refine the predictions to readily identify the best antigens that could mediate T-cell responses. Such…

Prof. Doron Gerber – An Integrated Microfluidics Approach for Personalized Cancer Drug Sensitivity and Resistance Assay

Abstract Cancer is the second leading cause of death globally. Matching proper treatment and dosage is crucial for a positive outcome. Any given drug may affect patients with similar tumors differently. Personalized medicine aims to address this issue. Unfortunately, most cancer samples cannot be expanded in culture, limiting conventional cell‐based testing. Herein, presented is a…

Dr. Orly Avni – Social-Stress-Responsive Microbiota Induces Stimulation of Self-Reactive Effector T Helper Cells

ABSTRACT Stressful life events are considered a risk factor for autoimmune disorders, though the mechanisms are unclear. Here we demonstrate that chronic social stress induces virulence-associated transcriptional patterns in the murine gut microbiota. The stress-influenced microbiota increased the presence of effector T helper cells in the mesenteric lymph nodes, including myelin-autoreactive cells. Inhibition of the…

Prof. Rachel Dekel – Adjustment profiles of Yom Kippur war veterans seeking delayed help from the IDF

Abstract The most common stress reactions following combat are post-traumatic stress reactions. These responses have a great impact on quality-of-life and can damage victims’ personal, familial, and social functioning. However, of the people who develop PTSD, only a few request psychological help. The first aim of the research was, therefore, to examine the long-term adjustment…

Dr. Hava Gil-Henn – L-Norvaline, a new therapeutic agent against Alzheimer’s disease

AbstractGrowing evidence highlights the role of arginase activity in the manifestation of Alzheimer’s disease (AD). Upregulation of arginase was shown to contribute to neurodegeneration. Regulation of arginase activity appears to be a promising approach for interfering with the pathogenesis of AD. Therefore, the enzyme represents a novel therapeutic target. In this study, we administered an…
Prof. Sol Efroni

Prof. Efroni Sol – Resistance to paclitaxel is associated with a variant of the gene BCL2 in multiple tumor types

Abstract Paclitaxel, the most commonly used form of chemotherapy, is utilized in curative protocols in different types of cancer. The response to treatment differs among patients. Biological interpretation of a mechanism to explain this personalized response is still unavailable. Since paclitaxel is known to target BCL2 and TUBB1, we used pan-cancer genomic data from hundreds…

Dr. Omry Koren – Progesterone Increases Bifidobacterium Relative Abundance during Late Pregnancy

Highlights Bifidobacterium abundance increases in the gut during pregnancy in women and mice Progesterone supplementation alters gut microbial composition in mice and in vitro Progesterone supplementation increases Bifidobacterium abundance in mice and in vitro We suggest that progesterone promotes Bifidobacterium growth during late pregnancy Summary Gestation is accompanied by alterations in the microbial repertoire; however, the mechanisms driving these changes are unknown. Here, we…

Dr. Evan Elliot – Antidepressants affect gut microbiota and Ruminococcus flavefaciens is able to abolish their effects on depressive-like behavior

Abstract Accumulating evidence demonstrates that the gut microbiota affects brain function and behavior, including depressive behavior. Antidepressants are the main drugs used for treatment of depression. We hypothesized that antidepressant treatment could modify gut microbiota which can partially mediate their antidepressant effects. Mice were chronically treated with one of five antidepressants (fluoxetine, escitalopram, venlafaxine, duloxetine…
Prof. Erez Levanon

Prof. Levanon Erez – Specific inhibition of splicing factor activity by decoy RNA oligonucleotides

Abstract Alternative splicing, a fundamental step in gene expression, is deregulated in many diseases. Splicing factors (SFs), which regulate this process, are up- or down regulated or mutated in several diseases including cancer. To date, there are no inhibitors that directly inhibit the activity of SFs. We designed decoy oligonucleotides, composed of several repeats of…

Prof. Rachel Dekel – The Interrelations of Physical and Mental Health: Self-Rated Health, Depression, and PTSD Among Female IPV Survivors

Abstract Researchers have found that intimate partner violence (IPV) is associated with low self-rated health (SRH), which is correlated with increased medication usage, and has tremendous social consequences. IPV and low SRH are associated with posttraumatic stress disorder (PTSD) and depression, and the current study examined the interrelations between these variables among 505 Israeli women…
Prof. Rachela Popovtzer

Prof. Popovtzer Rachela – Golden Exosomes Selectively Target Brain Pathologies in Neurodegenerative and Neurodevelopmental Disorders

Abstract Exosomes, nanovesicles that are secreted by different cell types, enable intercellular communication at local or distant sites. Alhough they have been found to cross the blood brain barrier, their migration and homing abilities within the brain remain unstudied. We have recently developed a method for longitudinal and quantitative in vivo neuroimaging of exosomes based…

Prof. Arnon Blum – Inhibition of Endothelial Progenitor Cells in the first 24 hours of an Acute Ischemic Cerebrovascular Event

Abstract Background: Endothelial progenitor cells may have a role in ongoing endothelial repair. Impaired mobilization or depletion of these cells may contribute to progression of vascular disease. Our hypothesis was that endothelial progenitor cells would be suppressed in patients with acute cerebrovascular event based on our previous study that found severe endothelial dysfunction in those patients.…

Dr. Meital Gal-Tanamy -Dysregulation of the cohesin subunit RAD21 by Hepatitis C virus mediates host–virus interactions

Abstract Hepatitis C virus (HCV) infection is the leading cause of chronic hepatitis, which often results in liver fibrosis, cirrhosis and hepatocellular carcinoma (HCC). HCV possesses an RNA genome and its replication is confined to the cytoplasm. Yet, infection with HCV leads to global changes in gene expression, and chromosomal instability (CIN) in the host…

Prof. Ron Unger – Unplanned Cesarean Delivery in the Second Stage of Labor Holds Higher Odds of Complications than in the First Stage, while Similar in Primiparas and Multiparas

Abstract Background: While endeavors to reduce cesarean delivery (CD) rates are given priority worldwide, it is important to evaluate if these efforts place parturients and neonates at risk. CD performed in the second stage of labor carries higher risks of maternal and fetal complications and is a more challenging surgical procedure than that performed in the…
Prof. Sol Efroni

Prof. Efroni Sol – The Immune System Computes the State of the Body: Crowd Wisdom, Machine Learning, and Immune Cell Reference Repertoires Help Manage Inflammation

Abstract Here, we outline an overview of the mammalian immune system that updates and extends the classical clonal selection paradigm. Rather than focusing on strict self-not-self discrimination, we propose that the system orchestrates variable inflammatory responses that maintain the body and its symbiosis with the microbiome while eliminating the threat from pathogenic infectious agents and…

Prof. Dror Fixler – Hyperlipidemic mice as a model for a real‐time in vivo detection of atherosclerosis by gold nanorods‐based diffusion reflection technique

Abstract Atherosclerosis (AS), the leading cause of morbidity and mortality in cardiovascular disease, needs an early detection for treatment and prevention of fatal events. Here, for the first time, we applied gold nanorods (GNRs)‐assisted diffusion reflection (DR), a noninvasive technique for in vivo detection of AS in a high‐fat‐diet‐induced c57bl mouse model, which resembles the…

Prof. Eitan Okun – Restoring microglial and astroglial homeostasis using DNA immunization in a Down Syndrome mouse model

Abstract Down Syndrome (DS), the most common cause of genetic intellectual disability, is characterized by over-expression of the APP and DYRK1A genes, located on the triplicated chromosome 21. This chromosomal abnormality leads to a cognitive decline mediated by Amyloid-β (Aβ) overproduction and tau hyper-phosphorylation as early as the age of 40. In this study, we used the Ts65Dn mouse…

Dr. Hava Gil-Henn – Commentary on Giralt et al.: PTK2B/Pyk2 overexpression improves a mouse model of Alzheimer’s disease

Abstract Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that is the most common cause of dementia and the 6th leading cause of death. Although research has revealed significant information about AD, much is yet to be discovered about the precise biological changes that cause AD and how the disease could be prevented, slowed, or stopped. Accumulating evidence…

Dr. Meital Gal-Tanamy – Antibody Repertoire Analysis of Hepatitis C Virus Infections Identifies Immune Signatures Associated With Spontaneous Clearance

Hepatitis C virus (HCV) is a major public health concern, with over 70 million people infected worldwide, who are at risk for developing life-threatening liver disease. No vaccine is available, and immunity against the virus is not well-understood. Following the acute stage, HCV usually causes chronic infections. However, ~30% of infected individuals spontaneously clear the…

Prof. Dror Fixler – Self calibration iso-pathlength point in cylindrical tissue geometry: solution of steady-state photon diffusion based on the extrapolated zero-boundary

Abstract Near-infrared optical techniques permit tissue diagnosis by surface measurement. However, the geometrical shape of this interface profiles the intensity of the surface measurement, which is found to have an iso-pathlength (IPL) point allowing for absorption identification independent of tissue scattering. The IPL point was projected in Monte Carlo (MC) simulation, validated experimentally in cylindrical…
Prof. Erez Levanon

Prof. Levanon Erez – Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade

Abstract Most patients with cancer either do not respond to immune checkpoint blockade or develop resistance to it, often because of acquired mutations that impair antigen presentation. Here we show that loss of function of the RNA-editing enzyme ADAR1 in tumour cells profoundly sensitizes tumours to immunotherapy and overcomes resistance to checkpoint blockade. In the…
Prof. Sol Efroni

Prof. Efroni Sol – Network Representation of T-Cell Repertoire— A Novel Tool to Analyze Immune Response to Cancer Formation

Abstract The T cell repertoire potentially presents complexity compatible, or greater than, that of the human brain. T cell based immune response is involved with practically every part of human physiology, and high-throughput biology needed to follow the T-cell repertoire has made great leaps with the advent of massive parallel sequencing [1]. Nevertheless, tools to…

Dr. Hava Gil-Henn – Plasma exosomes stimulate breast cancer metastasis through surface interactions and activation of FAK signaling

Abstract Purpose The interaction between malignant cells and surrounding healthy tissues is a critical factor in the metastatic progression of breast cancer (BC). Extracellular vesicles, especially exosomes, are known to be involved in inter-cellular communication during cancer progression. In the study presented herein, we aimed to evaluate the role of circulating plasma exosomes in the…

Dr. Dana Atzil-Slonim – Therapists’ Empathic Accuracy Toward Their Clients’ Emotions

Abstract Objective: Therapists’ empathic accuracy (EA) toward their clients’ fluctuating emotions is a crucial clinical skill that underlies many therapeutic interventions. In contrast to the subjective components of empathy, limited empirical work has addressed EA or its effect on the outcomes of psychotherapy. Here, we differentiate between the components of EA (tracking accuracy, directional discrepancy) as…

Prof. Ron Unger – Iatrogenic hypertension a bioinformatic analysis

Abstract It is well known that a myriad of medications and substances can induce side effects that are related to blood pressure (BP) regulation. This study aims to investigate why certain drugs tend to cause iatrogenic hypertension (HTN) and focus on drug targets that are implicated in these conditions. Databases and resources such as SIDER,…

Dr David Enshell-Seijffers – The Serine Protease Activity of Corin Is Required for Normal Pigment Type Switching

To the Editor The production and deposition of pigment in mouse skin are confined to the hair follicle and hair shaft, respectively. Hair pigmentation in mice involves the deposition of two types of pigment, black (eumelanin) and yellow (pheomelanin), in a very specific pattern (Barsh et al., 2000). Pigment, either black or yellow, is synthesized during…

Prof. Ron Unger – An Integrative Scoring System for Survival Prediction Following Umbilical Cord Blood Transplantation in Acute Leukemia

Abstract Purpose: Survival of acute leukemia (AL) patients following umbilical cord blood transplantation (UCBT) is dependent on an array of individual features. Integrative models for risk assessment are lacking. We sought to develop a scoring system for prediction of overall survival (OS) and leukemia-free survival (LFS) at 2 years following UCBT in AL patients. Experimental Design: The…

Prof. Ron Unger – Dietary patterns are positively associated with semen quality

Objective To study association of semen quality with a priori whole dietary pattern indexes, which reflect real-world dietary practices and the numerous combinations by which foods are consumed: Healthy Eating Index (HEI), Dietary Approaches to Stop Hypertension (DASH), alternate Mediterranean Diet score (aMED), and Alternative Healthy Eating Index (AHEI). Design A cross-sectional single-center study. Setting Hospital fertility…
Prof. Sol Efroni

Prof. Efroni Sol – Tamoxifen-resistant breast cancer cells are resistant to DNA-damaging chemotherapy because of upregulated BARD1 and BRCA1

Abstract Tamoxifen resistance is accountable for relapse in many ER-positive breast cancer patients. Most of these recurrent patients receive chemotherapy, but their chemosensitivity is unknown. Here, we report that tamoxifen-resistant breast cancer cells express significantly more BARD1 and BRCA1, leading to resistance to DNA-damaging chemotherapy including cisplatin and adriamycin, but not to paclitaxel. Silencing BARD1…

Prof. Ron Unger – Sonographic large fetal head circumference and risk of cesarean delivery

Background Background Persistently high rates of cesarean deliveries are cause for concern for physicians, patients, and health systems. Prelabor assessment might be refined by identifying factors that help predict an individual patient’s risk of cesarean delivery. Such factors may contribute to patient safety and satisfaction as well as health system planning and resource allocation. In an earlier…

Prof. Cyrille Cohen – Fast Image-Guided Stratification Using Anti- Programmed Death Ligand 1 Gold Nanoparticles for Cancer Immunotherapy

Abstract Cancer immunotherapy has made enormous progress in offering safer and more effective treatments for the disease. Specifically, programmed death ligand 1 antibody (αPDL1), designed to perform immune checkpoint blockade (ICB), is now considered a pillar in cancer immunotherapy. However, due to the complexity and heterogeneity of tumors, as well as the diversity in patient…

Prof. Ron Unger – Machine learning for prediction of 30-day mortality after ST elevation myocardial infraction An Acute Coronary Syndrome Israeli Survey data mining study

Abstract Background Risk scores for prediction of mortality 30-days following a ST-segment elevation myocardial infarction (STEMI) have been developed using a conventional statistical approach. Objective To evaluate an array of machine learning (ML) algorithms for prediction of mortality at 30-days in STEMI patients and to compare these to the conventional validated risk scores. Methods This was a…

Prof. Cyrille Cohen – The mutational status of p53 can influence its recognition by human T-cells

ABSTRACT p53 was reported to be an attractive immunotherapy target because it is mutated in approximately half of human cancers, resulting in its inactivation and often accumulation in tumor cells. Peptides derived from p53 are presented by class I MHC molecules and may act as tumor-associated epitopes which could be targeted by p53-specific T cells.…

Prof. Ron Unger – Validation of the acute leukemia-EBMT score for prediction of mortality following allogeneic stem cell transplantation in a multi-center GITMO cohort

Abstract Predictive models may help in determining the risk/benefit ratio of allogeneic hematopoietic stem cell transplantation (HSCT) in acute leukemia (AL). Using a machine‐learning algorithm we have previously developed the AL‐ European Society for Blood and Marrow Transplantation (EBMT) score for prediction of mortality following transplantation. We report here the first external validation of the…
The Dangoor Centre For Personalized Medicine

Kaposi’s sarcoma genetic predisposition and personalized therapy

Familial Kaposi’s sarcoma and genetic whole exome analysis Background: Kaposi’s sarcoma (KS) is a rare malignancy most commonly appearing in HIV carriers. In addition to HIV carriers, there are rare families with genetic predisposition for KS, some are Jewish descent from Morocco. Professor Moshe Schaffer identified a few such families in his service as oncologist…

First Dangoor Centre Research Grant Awarded

The first grant of the Dangoor Center for Personalized Medicine has been awarded to a project using innovative epigenetic methods in order to enhance diagnosis and treatment of patients with acute coronary disease. Cardiovascular diseases are defined by the patient’s loss of flexibility in his or her blood vessels or his or her obstructed and…

Prof. Ron Unger – Prediction of Allogeneic Hematopoietic Stem-Cell Transplantation Mortality 100 Days After Transplantation Using a Machine Learning Algorithm A European Group for Blood and Marrow Transplantation Acute Leukemia Working Party Retrospective Data Mining Study

Purpose Allogeneic hematopoietic stem-cell transplantation (HSCT) is potentially curative for acute leukemia (AL), but carries considerable risk. Machine learning algorithms, which are part of the data mining (DM) approach, may serve for transplantation-related mortality risk prediction.Patients and Methods This work is a retrospective DM study on a cohort of 28,236 adult HSCT recipients from the…